|
KMID : 1007520000090040239
|
|
Food Science and Biotechnology
2000 Volume.9 No. 4 p.239 ~ p.242
|
|
|
Effect of γ-Irradiation on the Molecular Properties of Egg White Proteins
|
|
Song Kyung-Bin
Moon Sun-Ae
|
|
|
Abstract
|
|
|
|
To elucidate the effect of oxygen radicals on the molecular properties of egg white proteins, the secondary and tertiary structure and molecular weight size of ovalbumin, ovomucoid, and ovotransferrin were examined after irradiation of proteins at various doses. Gamma-irradiation of protein solutions caused the disruption of the ordered structure of protein molecules as well as degradation, cross-linking, and aggregation of the polypeptide chains. Circular dichroism study showed that increase of radiation decreased the ordered structure of proteins. Fluorescence spectroscopy indicated that irradiation quenched the emission intensity excited at 280 nm. SDS-PAGE and gel permeation chromatography study indicated that radiation caused initial fragmentation of polypeptide chains and as a result subsequent aggregation due to cross-linking of protein molecules.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|